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KMID : 1024420130170040362
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Food Engineering Progress
2013 Volume.17 No. 4 p.362 ~ p.368
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Concentration of Rice Bran Lipid Soluble Bioactive Substances Using Supercritical Carbon Dioxide
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Kim In-Hwan
Yoon Sung-Won
Lee Jun-Soo
Lee Jeom-Sig
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Abstract
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The aim of the present study was to examine the effects of different pressure and temperature combinations on theconcentrations of tocols homologues, policosanol, phytosterol, and γ-oryzanol in crude rice bran oil (RBO) usingsupercritical carbon dioxide (SC-CO2). To effectively separate oily substances from bioactive substances, fatty acidethyl esterification was performed by sulfuric acid or the lipase catalytic method. In sulfuric acid esterification, γ-tocotrienol content was decreased by ca. 50%, whereas in lipase esterification, γ-tocotrienol content was notdecreased. Therefore, lipase catalytic esterification was performed for the fatty acid alkyl esterification of RBO. Thefractional extraction of RBO ethyl esters was performed using SC-CO2 in the condition of 9.62Mpa, 10.34MPa,or 11.03MPa and 45oC, 50oC, or 55oC. During the fractionation of RBO using SC-CO2, a little tocols content wasextracted with fatty acid ethyl esters, but policosanol and phytosterol were not extracted. Especially, there is a highcorrelation (R2=0.9306) between the density of CO2 and tocols contents extracted to the fractions. The concentrationrates of tocols and phytosterol were the highest in the condition of 55oC and 9.62MPa. Therefore, the lower CO2density applied, the more efficient the concentrations of tocols and phytosterol were in this study.
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KEYWORD
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rice bran oil, supercritical CO2 extraction, tocols, γ-oryzanol, lipase esterification
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